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KMID : 0613820020120060765
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Journal of Life Science
2002 Volume.12 No. 6 p.765 ~ p.768
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Optimization of Epoxide Hydrolase-Catalyzed Enantioselective Hydrolysis of Racemic Styrene Oxide
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Lee Eun-Yeol
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Abstract
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Enantioselective hydrolysis of racemic styrene oxide by Rhodotorula sp. CL-82 was investigated. Reaction conditions including pH, temperature, and volume ratio of organic cosolvent were optimized using response surface methodology, and the optimal conditions of pH, temperature, and the volume ratio of cosolvent were determined to be 7.64, 33.26℃, and 3.09 %(v/v), respectively. Chiral (S)-phenyl oxirane could be obtained with high enantiomeric purity (ee > 99%) and 20% yield (theoretical yield = 50%) at the optimal condition.
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KEYWORD
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enantioselective hydrolysis, Rhodotorula sp. CL-82, styrene oxide, response surface methodology
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